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Nikon Ti2 研究級(jí)倒立顯微鏡(全系列)
Nikon Eclipse Ti2提供了前所未見(jiàn)的大視野範(fàn)圍(Field of view=25mm)，讓您的科研級(jí)sCMOS相機(jī)視野範(fàn)圍不再大材小用，且讓高通量擷取影像更快速。因應(yīng)對(duì)超高解析影像 (Super resolution)的顯微鏡需求，Nikon Ti2更特別加強(qiáng)了穩(wěn)定性，降低影像飄移(drift)，且大幅提升了軟體對(duì)於控制硬體的功能，來(lái)達(dá)到高速影像的要求。另外Nikon Ti2獨(dú)特的智能感知導(dǎo)引功能，透過(guò)圖像化的介面，當(dāng)操作錯(cuò)誤時(shí)能及時(shí)提醒使用者。為了實(shí)驗(yàn)的重現(xiàn)性及品質(zhì)，每個(gè)感知元件皆能在擷取影像中自動(dòng)記錄，再搭配Nikon強(qiáng)大的擷取及分析軟體Nikon NIS-Elements，全新革命性的影像體驗(yàn)就此展開(kāi)。
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★Nikon Ti2研究級(jí)倒立顯微鏡擁有三種機(jī)型：
◆Ti2-U手動(dòng)機(jī)型 ◆Ti2-A手動(dòng)智能感知 ◆Ti2-E電動(dòng)智能感知
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★Nikon Ti2研究級(jí)倒立顯微鏡產(chǎn)品優(yōu)勢(shì)：
Ａ.突破性的視野範(fàn)圍：
當(dāng)市面上的大片幅sCMOS影像擷取系統(tǒng)陸續(xù)推出，傳統(tǒng)的18mm視野範(fàn)圍無(wú)法放入所有範(fàn)圍的影像而犧牲掉邊界的影像，且影像邊緣會(huì)出現(xiàn)暗角。為了改進(jìn)上述缺點(diǎn)，Nikon推出Ti2系列，使用者可真正利用的25mm拍照大視野範(fàn)圍，提升了觀察尺度。另外，較大的視野讓您花較少的時(shí)間，提升了拼圖速度，滿足更快的影像擷取以及高通量分析的需求。
▲Single FOV of cultured neurons, captured with a CFI Plan Apo lambda 60x objective and DS-Qi2 camera.
Photo courtesy of J. Rappoport, Nikon Imaging Center, Northwestern Univ.; Sample courtesy of S. Kemal, B. Wang, and R. Vassar, Northwestern Univ.
A-1: 提升全視野的明亮度
在拍攝高倍DIC或是拼圖的過(guò)程中，是否曾受到亮度不均而感到困擾？
Nikon Ti2搭配Fly-eye技術(shù)，提供高功率的LED燈源，確保在大視野範(fàn)圍觀察下的亮度平均。
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螢光的大視野範(fàn)圍觀察也兼顧到了亮度，Ti2可選購(gòu)搭配石英fly-eye鏡片，提供全波段光譜包含紫外光更高的穿透率。
更大直徑的螢光濾片組，加厚鍍膜的濾片，提高更好的訊雜比。
 大視野螢光光源 |  大尺寸螢光濾片 |
Ａ-2. 直徑加大的觀察模式
為了因應(yīng)25mm大視野的成像孔洞，觀察光路直徑也隨之增加。比起傳統(tǒng)顯微鏡幾乎可增加2倍的視野範(fàn)圍，讓使用者搭配大感光元件CMOS時(shí)能有的表現(xiàn)。 增擴(kuò)直徑後的光路鏡片 |  超大視野範(fàn)圍25mm的分光孔 |
Ａ-3. 提供更大視野的物鏡組
Nikon Apo的物鏡有優(yōu)異的平場(chǎng)校正功能提供高品質(zhì)的影像，Apo OFN25系列提供較大的視野範(fàn)圍讓使用者能更快獲得影像。
*OFN 25=Objective Field Number 25，可搭配視野範(fàn)圍為25mm的目鏡
Ａ-4. 大視野相機(jī)
Nikon擁有多年單眼數(shù)位相機(jī)的研發(fā)技術(shù)經(jīng)驗(yàn)，提供1625萬(wàn)畫(huà)素CMOS感光元件的大片幅相機(jī)，能搭配Ti2的25mm的視野範(fàn)圍。 DS-Qi2 單色高感光螢光相機(jī) |  DS-Ri2 彩色高速相機(jī) |
Ｂ.的Nikon光學(xué)系統(tǒng)
Nikon的高精確性CFI60無(wú)限遠(yuǎn)光學(xué)系統(tǒng)，帶給使用者更清晰的影像。
B-1. Apodized Phase Contrast 相位差模式
Nikon的相位差模式，相較於傳統(tǒng)相位差效果，更加強(qiáng)對(duì)比及減少假光。
▲B(niǎo)SC-1 cells captured with CFI S Plan Fluor ELWD ADM 40xC objective
▲裝置在APC物鏡的相位環(huán)示意圖
B-2 DIC 微分干涉相位差
Nikon考量使用DIC影像需要高解析度和高對(duì)比的可見(jiàn)光細(xì)胞表面影像，因此設(shè)計(jì)出每顆DIC物鏡皆有搭配專(zhuān)屬的DIC模組片，達(dá)到品質(zhì)的效果。
Ｂ-3 NAMC (Nikon Advanced Modulation Contrast)
Nikon技術(shù)可用塑膠底容器即可帶有立體感成像，尤其是透明樣本及卵子。NAMC可提供3D的立體效果，對(duì)比度則可簡(jiǎn)單的被調(diào)整。
NAMC/20x
B-4 螢光影像技術(shù)
Nikon λ系列物鏡使用奈米晶體鍍膜技術(shù)，降低光線的反射，提高螢光的穿透率。對(duì)於低螢光訊號(hào)的樣本和需多色螢光激發(fā)的樣本的觀察有極大幫助。再搭配Ti2新型的濾片，對(duì)於目前單分子影像及光激發(fā)應(yīng)用實(shí)驗(yàn)上更有強(qiáng)化效果。
C：Intelligent Assist Guide 智能協(xié)助指引 (Ti2-E/A)
有了智能協(xié)助指引幫助使用者無(wú)須記住冗長(zhǎng)的校正及操作步驟，減少使用上的錯(cuò)誤，維持實(shí)驗(yàn)的再現(xiàn)性。
C-1 顯微鏡狀態(tài)的連續(xù)展現(xiàn)
透過(guò)內(nèi)建的感知偵測(cè)器，所有當(dāng)您在擷取影像的資訊皆被記錄下來(lái)，因此可以輕鬆地得知實(shí)驗(yàn)操作的參數(shù)及操作錯(cuò)誤通知。除此之外，內(nèi)建感知的照相機(jī)可提供相位差、DIC、雷射校正引導(dǎo)。
▲發(fā)光面板方便於暗室使用
C-2 操作引導(dǎo)精靈
實(shí)驗(yàn)進(jìn)行中但顯微鏡有校正或操作問(wèn)題怎麼辦？
新的Ti2提供操作引導(dǎo)精靈幫助使用者即時(shí)除錯(cuò)導(dǎo)引與校正提示。
提供步驟的引導(dǎo)教學(xué)，幫助使用者自行進(jìn)行校正程序，可透過(guò)電腦或是平板來(lái)觀看校正介面，進(jìn)而連線顯微鏡校正。
▲透過(guò)平板螢?zāi)贿M(jìn)行光路校正
▲透過(guò)除錯(cuò)偵測(cè)驚嘆號(hào)，得知修正步驟
★Nikon Ti2 specifications規(guī)格表
ECLIPSE Ti2-E / Ti2-E/B *1 | ECLIPSE T2-A | ECLIPSE T2-U | ||
Main body | Optical system | Infinity-corrected CFI60 | ||
Field number*2 | 22 with C-mount, 25 with F-mount | |||
Intermediate Magnification switching | Manual switching of 1.0x/1.5x (exchangeable from 1.5x to 2.0x) | |||
Status detection | - | |||
Bertrａnd lens | Manual in/out, and manual focus, Status detection | - | ||
Output port | 4 Motorized positions Eyepiece 99%, left 99%, right 99%,eyepiece 20%/left 80% (Ti2-E/B: bottom 99%) | 4 Manual positions Eyepiece 99%, left 99%, right 99%, option(to eyepiece 20%/left 80% or eyepiece 20%/right 80%) | ||
Can add ports by use of back port unit and/or choice of tube base unit*3 | ||||
Focusing unit | Motorized drive, Coarse/fine focus changeover, 10mm stroke Minimum increments: 0.01um, 0.02um (with encoder control) | Manual drive, Coarse/fine focusing knob, 10mm stroke | ||
Stage up | Available *4 | |||
Tube body | Binocular tube | Binocular S tube TC-T-TS (field number 22), Ergonomic ER tube TC-T-ER (field number 22) | ||
Motorized eyepiece tube base unit for external PH (TI2-T-BP-E) | Camera port (field number 16), Motorized PH turret with 4 motorized positions | - | ||
Assist eyepiece tube base unit (TI2-T-BA) | Assist camera (field number 22), Status detection | - | ||
Eyepiece tube base unit with port (TI2-T-BC) | Camera port (field number 16) | - | Camera port(field number 16) | |
Transmitted illumination | Pillar for transmitted illumination (TI2-D-PD) | Condenser vertical stroke: 66mm, Backward tilting up to 25 degrees, With field diagram and refocusing mechanism 2 filter slot positions (4 filter position option is also available with Filter Slider for transmitted illumination (TI2-D-SF) | ||
LED Lamphouse for dia illumination (TI2-D-LHLED) | High power LED | |||
Precentered Lamphouse (D-LH/LC) | 100W halogen bulb (pre-centered) | |||
Condenser | Motorized condenser turret (TI2-C-TC-E) | 7 motorized positions (ψ37mm x4,ψ39mm x3), LWD/ELWD/CLWD/NAMC condenser lenses are supported | - | |
Intelligent condenser turret (TI2-C-TC-I) | 7 manual positions (ψ37mm x4,ψ39mm x3), Status detection, LWD/ELWD/CLWD/NAMC condenser lenses are supported | - | ||
Condenser turret (TC-C-TC) | 7 manual positions (ψ37mm x4,ψ39mm x3), LWD/ELWD/CLWD/NAMC condenser lenses are supported | |||
ELWD-S condenser turret (TE-C) | 4 manual positions, With ELWD condenser lens (NA0.3 / OD65) | |||
HNA condenser slider (TI2-C-SCH) | 2 manual positions (ψ37mm x1,ψ39mm x1), HNA dry lens/ HNA oil lens are supported | |||
Condenser lens | LWD (W.D.=30mm, NA=0.52), ELWD (W.D.=75mm, NA=0.3), CLWD (W.D.=13mm, NA=0.72), HNA dry (W.D.=5mm, NA=0.85), HNA oil (W.D.=1.9mm, NA=1.3), NAMC (W.D.=44mm, NA=0.4) | |||
Stage | Motorized stage (TI2-S-SE-E, TI2-S-SS-E) | Stroke X:±57mm, Stroke Y:±36.5mm, Max drive speed: approx. 25mm/sec, Magnetic sample holder | - | |
Stage (TC-S-SR, TC-S-SRF) | Stroke X:±57mm, Stroke Y:±36.5mm, Adjustable stroke range (3 levels) with adjusting pin, Long/middle/short handle options available | |||
Gliding stage (TC-S-GS) | Strokeψ20mm | |||
Nosepiece | Perfect Focus Unit with motorized nosepiece for Auto Correction Collar (TI2-N-NDA-P) | 5 motorized positions, Simple waterproof structure | - | |
Motorized DIC sextuple nosepiece (TI2-N-ND-E) Perfect Focus Unit with motorized nosepiece (TI2-N-ND-P) Perfect Focus Unit with motorized nosepiece for MP (TI2-N-NDM-P) | 6 motorized positions, Simple waterproof structure | - | ||
Intelligent DIC sextuple nosepiece (TI2-N-ND-I) | 6 manual positions, Status detection, Simple waterproof structure | - | ||
Sextuple nosepiece (TI2-N-N), DIC sextuple nosepiece (TI2-N-ND) | 6 manual positions, Simple waterproof structure | |||
Epi-filter turret | Motorized epi filter turret (TI2-F-FLT-E, TI2-F-FLTH-E) | 6 motorized positions, Motorized shutter | - | |
Intelligent epi filter turret (TI2-F-FLT-I) | 6 manual positions, Manual shutter, Status detection*5 | |||
Filter wheel/ shutter | Motorized BA filter wheel (TI2-P-FWB-E) | 7 motorized positions, High speed mode: 50ms, Low vibration mode: 100ms (movement time between adjacent positions) | - | |
Motorized shutter (NI-SH-E)*6 | 12ms to open/close | |||
Epi-fluorescence attachment | EPI-FL module (TI2-LA-FL), EPI-FL module for large FOV (TI2-LA-FLL) | Supports fiber illuminator; includes 2-position filter slider and aperture diaphragm | ||
Simple EPI-FL attachment (TI2-F-FLS) | Supports both fiber illuminator and lamp house; includes 3-position filter slider | |||
Field stop slider | Circular (TI2-F-FSC), rectangular (TI2-F-FSR), square (Ti2-F-FSS) aperture options | |||
Control unit | Controller, display device | Stage joystick (TI2-S-JS), Tablet | Tablet | - |
Controller for TI2-E (TI2-CTRE) | USB/LAN interface, I/O function | - | ||
Operating environmental | Temperature: 0℃-40℃, Humidity: 60% RH max. (at +40℃, no condensation), Indoor use only | |||
*1 Motorized model with a bottom port *2 Limitations apply based on objective and filter cube choice, stage-up configuration, and illumination module, etc. *3 Tube base units with a port cannot be used with Ti2-A *4 Stage up kit is required. Please contact Nikon. *5 Status detection cannot be used when attached to the Ti2-U *6 NI-SH-CON Controller for Motorized Shutter is required for use with the Ti2-A/Ti2-U | ||||
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